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sideFadeLine
Rag1 Knockout Rat
Model Detail >
Blue Fade Line
Nomenclature: SD- Rag1 tm1sage
Genotype: Homozygous
Product number: TGRS3920
Research Applications
  • Xenograft
  • Cancer metastasis
  • Vaccine development
  • Inflammation/Autoimmune disorders
  • Thrombosis/Cardiac fibrosis
  • Vascular defects
  • Hematopoieses
  • Infectious disease

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Description

Recombination Activation Genes (Rag) encode enzymes that play an important role in the rearrangement and recombination of the genes of immunoglobulin and T cell receptor molecules during the process of V(D)J recombination. Rag1 knockout rats lack mature B and T lymphocytes.

Characteristics
  • 29 bp deletion within Exon 2 on chromosome 3
  • Homozygous Rag1 knockout rats display loss of RAG1 protein via Western blot
  • Homozygous Rag1 knockout rats show loss of B and T cells by FACS analysis
  • Background Strain: Sprague-Dawley

Figure 1. Flow cytometric analysis of T cell subsets (CD4 and CD8), B cells and NK cells from wild type and Rag1 (-/-) knockout blood samples
The top panels represent wild type female and male rats. The lower panels represent samples from female and male Rag1 (-/-) knockout rats. Blood samples were collected from 4-, 10- and 20-week-old rats.

Recommended Diet

Normal rat chow

Additional Information

Mature B and T cells are critical components for an adaptive immune system. Rats deficient in Rag1 protein produce no mature B or T cells. This non-leaky model for severe combined immune deficiency is useful for vaccine development, as well as the study of autoimmune and infectious diseases.